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transcript accession number: nm_017758.4  (Biotechnology Information)

 
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    Biotechnology Information transcript accession number: nm_017758.4
    Patient demographics and tumor characteristics and association of <t> ALKBH5 </t> levels with clinicopathological features.
    Transcript Accession Number: Nm 017758.4, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcript accession number: nm_017758.4/product/Biotechnology Information
    Average 90 stars, based on 1 article reviews
    transcript accession number: nm_017758.4 - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma"

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-84352-w

    Patient demographics and tumor characteristics and association of ALKBH5 levels with clinicopathological features.
    Figure Legend Snippet: Patient demographics and tumor characteristics and association of ALKBH5 levels with clinicopathological features.

    Techniques Used: Expressing

    ( A ) qRT-PCR and Western blot were used to detect the expression of ALKBH5 to screen PTC cells. ( * P < 0.05 indicates a significant difference between each group compared to the Nthy-ori3-1 group). ( B ) Subcellular Localization of ALKBH5 Protein Detected by Immunofluorescence.
    Figure Legend Snippet: ( A ) qRT-PCR and Western blot were used to detect the expression of ALKBH5 to screen PTC cells. ( * P < 0.05 indicates a significant difference between each group compared to the Nthy-ori3-1 group). ( B ) Subcellular Localization of ALKBH5 Protein Detected by Immunofluorescence.

    Techniques Used: Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence

    Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells; B . TPC-1 cells).
    Figure Legend Snippet: Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells; B . TPC-1 cells).

    Techniques Used: Transfection, Biomarker Discovery, Over Expression

    Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells; B . Transwell assay to assess the invasion of IHH4 cells; C . Transwell assay to assess the invasion of TPC-1 cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).
    Figure Legend Snippet: Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells; B . Transwell assay to assess the invasion of IHH4 cells; C . Transwell assay to assess the invasion of TPC-1 cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

    Techniques Used: CCK-8 Assay, Transwell Assay

    m6A Methylation detection (A for IHH4 Cells; B for TPC-1 Cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).
    Figure Legend Snippet: m6A Methylation detection (A for IHH4 Cells; B for TPC-1 Cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

    Techniques Used: Methylation



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